Pig BDNF ELISA KIT (1 x 96 wells)

CAT#: EA102785

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Pig porcine BDNF. 96wells/kit, with removable strips.

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Pig BDNF
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 31.2pg/ml-2000pg/ml
• Specificity: This kit is used for quantitative detection of Pig BDNF
• Sensitivity: <15pg/ml
• Reactivities: Pig
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti-pig BDNF antibody 1
  • Lyophilized recombinant pig BDNF standard 10 ng/tube x 2
  • Biotinylated anti-pig BDNF antibody 100ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) 100ul (dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
  • Plate Sealers 4 x
  • Wash Buffer (25x) 20 ml
• Background: Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult Pig porcine brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding Pig porcine BDNF to chromosome 11, band p13.
• Gene Symbol: BDNF
• Gene ID: 397495