Human TREM2 ELISA KIT (1 x 96 wells)

CAT#: EA102484

For quantitative detection of human TREM2 in cell culture supernates, serum and plasma (heparin, EDTA).

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human TREM2
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 31.2 pg/ml - 2000 pg/ml
• Specificity: This kit is used for quantitative detection of Human TREM2
• Sensitivity: <10pg/ml
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti-human TREM2 antibody | 1
  • Lyophilized recombinant human TREM2 standard | 10 ng/tube x 2
  • Biotinylated anti-human TREM2 antibody | 100 ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Plate Sealers 4 x
  • Wash Buffer (25x) 20 ml
• Background: Triggering receptor expressed on myeloid cells 2(TRM2) encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms.
• Gene Symbol: TREM2
• Gene ID: 54209