Human BMP9 (GDF2) ELISA KIT (1 x 96 wells)

CAT#: EA102396

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For Quantitative Detection of human BMP-9 in bone tissue, cell culture supernates, serum plasma (heparin, EDTA) and urine.



USD 550.00


Availability*
3 Weeks

Size
    • 1 x 96 wells

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Specifications

Product Data
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Human GDF2
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 15.6pg/ml-1000pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA), urine, bone tissue.
Specificity This kit is used for quantitative detection of Human GDF2
Sensitivity <10pg/ml
Reactivities Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • 96-well plate precoated with anti-human GDF2/BMP9 antibody | 1
  • Lyophilized recombinant human GDF2/BMP9 standard | 10 ng/tube x 2
  • Biotinylated anti-human GDF2/BMP9 antibody | 100 ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12 ml
  • ABC diluent buffer | 12 ml
  • TMB color developing agent | 10 ml
  • TMB stop solution | 10 ml
  • Plate Sealers | 4
  • Wash Buffer (25x) | 20 ml

Background Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP)-9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.
Gene Symbol GDF2
Gene ID 2658
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