Human alpha Defensin 1 (DEFA1) ELISA KIT (1 x 96 wells)

CAT#: EA102360

For quantitative detection of human DEFA1 in cell culture supernates, serum and plasma (heparin, EDTA).


USD 913.00

3 Weeks*

Size
    • 1 x 96 wells

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Specifications

Product Data
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Human DEFA1
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 312pg/ml-20000pg/ml
Specificity This kit is used for quantitative detection of Human DEFA1
Sensitivity <10pg/ml
Reactivities Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Components
  • 96-well plate precoated with anti- human DEFA1 antibody | 1
  • Lyophilized recombinant human DEFA1 standard | 10ng/tubex2
  • Biotinylated anti- human DEFA1 antibody | 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
Background Defensin is a human protein that is encoded by the DEFA1 gene. Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation.
Gene Symbol DEFA1
Gene ID 1667

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