Human CD47 ELISA KIT (1 x 96 wells)

CAT#: EA102299

For quantitative detection of human CD47 in cell culture supernates and serum.

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich ELISA kit of Quantitative Detection for human CD47
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 125pg/ml-8000pg/ml
• Specificity: This kit is used for quantitative detection of human CD47
• Sensitivity: <10pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Components:
  • 96-well plate precoated with anti- human CD47 antibody | 1
  • Lyophilized recombinant human CD47 standard | 10 ng/tube×2
  • Biotinylated anti- human CD47 antibody | 100 μl (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 100 μl (dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12 ml
  • ABC diluent buffer | 12 ml
  • TMB color developing agent | 10 ml
  • TMB stop solution | 10 ml
  • Wash Buffer (25x) | 20 ml
  • Adhesive cover | 4
• Background: CD47 (Cluster of Differentiation 47), also known as integrin associated protein (IAP), is a transmembrane protein that in humans is encoded by the CD47 gene. It belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. Also CD47 is ubiquitously expressed in human cells and has found to be overexpressed in many different tumor cells.
• Gene Symbol: CD47
• Gene ID: 961