Mouse Ereg ELISA KIT (1 x 96 wells)

CAT#: EA102280

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For quantitative detection of mouse Epiregulin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

USD 550.00

3 Weeks

    • 1 x 96 wells

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Product Data
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for mouse Epiregulin
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 125pg/ml-8000pg/ml
Specificity This kit is used for quantitative detection of mouse Epiregulin
Sensitivity <10pg/ml
Reactivities Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
  • 96-well plate precoated with anti- mouse Epiregulin antibody | 1
  • Lyophilized recombinant mouse Epiregulin standard | 10ng/tube×2
  • Biotinylated anti- mouse Epiregulin antibody | 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Adhesive cover | 4
Background Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.
Gene Symbol Ereg
Gene ID 13874
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