Zika Virus (ZIKV) Real Time RT-PCR Kit

CAT#: EA102173

Zika Virus (ZIKV) Real Time RT-PCR Kit


 Product Datasheet for 'EA102173'

USD 399.00


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    • RT-PCR kit

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Specifications

Product Data
Description Zika Virus (ZIKV) Real Time RT-PCR Kit
Size RT-PCR kit
Format 25 test
Assay Type RT-PCR
Assay Length 6 hours
Signal fluorescent
Curve Range LOQ: 2 x 10^3 to 1 x 10^8 copies/ml
Sample Type RNA extract
Sample Volume 5 ul RNA extract/rxn
Specificity This kit is used for quantitative detection Zika virus in human serum
Sensitivity 1×10^3copies/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant viruses.
Interference No significant interference observed with available related molecules.
Components
  • 1. ZIKV Super Mix, 1 vial, 480 µl
  • 2. RT-PCR Enzyme Mix, 1 vial, 28 µl
  • 3. Molecular Grade Water, 1 vial, 400 µl
  • 4. Internal Control (IC), 1 vial, 30 µl
  • 5. ZIKV Positive Control (1×10^7copies/ml), 1 vial, 30 µl
Background The principle of the real-time detection is based on the fluorogenic 5’nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially is proportional to the amount of the specific PCR product. Monitoring the fluorescence intensities in real-time allows the detection of the accumulating product without having to re-open the reaction tube after the amplification.
Gene Symbol zika
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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