CA 19-9 ELISA Kit

CAT#: EA101115

CA 19-9 ELISA Kit

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hour
• Signal: Colorimetric
• Sample Type: Cell culture supernatant, serum, plasma
• Sample Volume: 10 ul
• Specificity: Natural and recombinant human CA 19-9
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components: See the product information for more details
• Background: The CA 19-9 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a mouse monoclonal antibody directed towards a unique antigenic site of the CA 19-9 molecule. An aliquot of specimen sample containing endogenous CA 19-9 is incubated in the coated well with assay buffer. After a washing step a second incubation follows with enzyme conjugate, which is an anti-CA 19-9 antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of CA 19-9 in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CA 19-9 in the specimen sample.