Human Varicella-Zoster-Virus (VZV) IgM ELISA kit

CAT#: EA100968

Human Varicella-Zoster-Virus (VZV) IgM ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Sample Type: Serum
• Sample Volume: 10 µl/well
• Specificity: This kit is used for quantitative detection human Varicella-Zoster-Virus (VZV) IgM
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwells coated with VZV antigen: 12x8x1
  • 2. Sample Diluent: 1 bottle (ready to use): 22 ml
  • 3. Calibrator: 1 Vial (ready to use): 1ml
  • 4. Positive Control: 1 vial (ready to use): 1ml
  • 5. Negative Control: . 1 vial (ready to use): 1ml
  • 6. Enzyme conjugate: 1 bottle (ready to use): 12ml
  • 7. TMB Substrate: 1 bottle (ready to use): 12ml
  • 8. Stop Solution: 1 bottle (ready to use): 12ml
  • 9. Wash concentrate 20X: 1 bottle: 25ml
• Background: Varicella zoster virus causes chickenpox a highly contagious disease acquired by touching the blisters or respiratory secretions, or through the air. A person is usually infectious 1-2 days before the rash to 4-5 days after the start of the rash, or until the blisters have formed crusts. Symptoms start about 2-3 weeks after exposure and include fever, tiredness, and an itchy rash with small blisters that dry up and form scabs in 2-4 days. More severe but rare problems or complications that could occur are pneumonia (especially in adults), skin infection, blood infection and encephalitis. Approximately 90% of chickenpox cases are in children 1-14 years of age, and 90% of people have had chickenpox by their early 20's. The reactivated form (herpes zoster: shingles) of VZV infection generally occurs in older adults whose immunity has waned, in infants or children exposed to VZV in the perinatal period or in the immunocompromised. VZV infection during pregnancy infrequently leads to maternal pneumonia. Chickenpox can occur during pregnancy in women seropositive for VZV, especially when seropositive at low titer, with low-avidity, largely IgG3 antibodies. Maternal VZV infection during pregnancy (especially between 13-20 weeks gestation) can be associated with outcomes ranging from skin scarring or limb hypoplasia to multi system involvement and death. Because VZV and herpes simplex virus (HSV) can cross-react, viral culture can be used to detect and differentiate HSV from VZV, but PCR testing may prove the most valuable for diagnosing and differentiating active infection. IgG antibodies can be detected 9 days after the onset of rash in varicella, 10 days in zoster; immunoreactivity peaks at an average 66 and 27 days, respectively. The IgM response to varicella is detected at 6-7 days post-onset and peaks at an average 14 days; IgM response to zoster is detectable at 8- 10 days and peaks at 18-19 days.