Human Alpha 1-Antichymotrypsin (ACT) ELISA kit

CAT#: EA100879

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Human Alpha 1-Antichymotrypsin (ACT) ELISA kit



USD 506.00


Availability*
3 Weeks

Size
    • 1 x 96 wells

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Specifications

Product Data
Description Human Alpha 1-Antichymotrypsin (ACT) ELISA kit
Size 1 x 96 wells
Format 8x12 divisible strips
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Curve Range 6.25-400 ng/mL
Sample Type Serum
Sample Volume 10 µl/well
Specificity This kit is used for quantitative detection human Alpha 1-Antichymotrypsin (ACT)
Sensitivity 6.25 ng/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 1. Microwell plate coated with anti-ACT Polyclonal Ab: 12x8x1
  • 2. Alpha 1-Antichymotrypsin Standard: 8 vials (ready to use): 0.2ml
  • 3. Anti-ACT Enzyme Conjugate: 1 vial (ready to use): 12ml
  • 4 Incubation Buffer: 1 bottle (ready to use): 12ml
  • 5. Sample Diluent: 3 bottles: 3x22ml
  • 6. TMB Substrate: 1 bottle (ready to use): 12ml
  • 7. Stop Solution: 1 bottle (ready to use): 12ml
  • 8. 20X Wash concentrate: 1 bottle: 25ml
Background The Alpha 1-Antichymotrypsin (ACT) kit is a solid phase direct ELISA sandwich method. The standards, samples and controls are added into designated wells, coated with anti-ACT polyclonal antibody, along with the incubation buffer. After a simple washing step, an anti-ACT enzyme conjugate reagent is added into each well. After the excess enzyme conjugate is washed out, the substrate is added into each well. Upon the addition of the substrate, the intensity of color developed is directly proportional to the concentration of ACT in the samples. A standard curve is generated relating color intensity to the concentration of ACT.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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