Human Alpha 1-Antichymotrypsin (ACT) ELISA kit

CAT#: EA100879

Human Alpha 1-Antichymotrypsin (ACT) ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 6.25-400 ng/mL
• Sample Type: Stool
• Sample Volume: 10 µl/well
• Specificity: This kit is used for quantitative detection human Alpha 1-Antichymotrypsin (ACT)
• Sensitivity: 6.25 ng/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwell plate coated with anti-ACT Polyclonal Ab: 12x8x1
  • 2. Alpha 1-Antichymotrypsin Standard: 8 vials (ready to use): 0.2ml
  • 3. Alpha 1-Antichymotrypsin Controls: 2 vials: 0.2ml
  • 4. Anti-ACT Enzyme Conjugate: 1 vial (ready to use): 12ml
  • 5. Incubation Buffer: 1 bottle (ready to use): 12ml
  • 6. Sample Diluent: 3 bottles: 3x22ml
  • 7. TMB Substrate: 1 bottle (ready to use): 12ml
  • 8. Stop Solution: 1 bottle (ready to use): 12ml
  • 9. 20X Wash concentrate: 1 bottle: 25ml
• Background: The Alpha 1-Antichymotrypsin (ACT) kit is a solid phase direct ELISA sandwich method. The standards, samples and controls are added into designated wells, coated with anti-ACT polyclonal antibody, along with the incubation buffer. After a simple washing step, an anti-ACT enzyme conjugate reagent is added into each well. After the excess enzyme conjugate is washed out, the substrate is added into each well. Upon the addition of the substrate, the intensity of color developed is directly proportional to the concentration of ACT in the samples. A standard curve is generated relating color intensity to the concentration of ACT.