Human Adrenocorticotropic hormone (ACTH) ELISA kit

CAT#: EA100848

Human Adrenocorticotropic hormone (ACTH) ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 6.4-483 pg/ml
• Sample Type: Serum
• Sample Volume: 200 µl/well
• Specificity: This kit is used for quantitative detection human Adrenocorticotropic hormone (ACTH)
• Sensitivity: 6.4 pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • Microwells coated with Streptavidin: 12x8x1
  • Biotinylated ACTH Antibody (Reagent 1): 2.7 ml
  • Peroxidase (Enzyme) labeled ACTH Antibody (1 Vial): 2.7 ml
  • Wash Concentrate (1 Vial): 30 ml
  • TMB Substrate (1 Vial): 15 ml
  • Stop Solution (1 Vial): 20 ml
  • Calibrators (5 Vials): 2 ml
  • Zero Calibrator (1 Vial): 4 ml
  • Controls 1 & 2 (CTRL) (2 Vials): 2 ml
• Background: Adrenocorticotropic Hormone (ACTH) is a 39-amino acid peptide hormone (MW = 4500) secreted by the pituitary to regulate the production of steroid hormones by the adrenal cortex. ACTH increases the synthesis and release of all adrenal sterioids, aldosterone, cortisol and adrenal androgens. It is the principal modulator of cortisol, the most important glucocorticoid in man. As the cortisol level in blood increases, release of ACTH is inhibited directly at the pituitary level. Through this same mechanism, decreasing cortisol levels lead to elevated ACTH levels. In healthy individuals, ACTH reaches a peak in the early morning (6:00 - 8:00 hour) and levels become lowest late in the day and near the beginning of the sleep period. Stress may also override the diurnal variation. Plasma ACTH assays are useful in the differential diagnosis of pituitary Cushing’s disease, Addison’s disease, autonomous ACTH producing pituitary tumors (e.g. Nelson’s syndrome), hypopituitarism with ACTH deficiency and ectopic ACTH syndrome. Primary adrenocortical insufficiencies, Addison’s disease. Hypopituitarism with ACTH deficiency, which is secondary adrenocortical insufficiency, is characterized by low plasma ACTH and cortisol concentrations, and a subnormal, but usually distinct adrenal response to stimulation with synthetic ACTH (Cortrosyn)