Human Triiodothyronine, Free (fT3) ELISA kit

CAT#: EA100839

Human Triiodothyronine, Free (fT3) ELISA kit

 Product Datasheet for 'EA100839'

USD 255.00

3 Weeks

    • 96-Well

Product images


Product Data
Description Human Triiodothyronine, Free (fT3) ELISA kit
Size 96-well
Format 8x12 divisible strips
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Curve Range 1.4-20 pg/ml
Sample Type Serum
Sample Volume 50 µl/well
Specificity This kit is used for quantitative detection human Triiodothyronine, Free (FT3)
Sensitivity 1.4 pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
  • 1. Microwells coated with Streptavidin: 12x8x1
  • 2. fT3 Standard: 6 vials ( ready to use): 0.5 ml
  • 3. Anti-T3 Biotin Solution: 7 ml
  • 4. fT3 Enzyme conjugate: 1 Bottle (ready to use): 7 ml
  • 5. TMB Substrate: 1 bottle (ready to use): 12 ml
  • 6. Stop Solution: 1 bottle (ready to use): 12 ml
  • 7. 20X Wash concentrate: 1 bottle: 25 ml
Background Over 99% of Triiodothyronine (T3) circulates in blood is bound to carrier proteins; thyroxine-binding globulin (TBG). However, only the free (unbound) portion of T3 is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alters, the total T3 level changes so that the freeT3 concentration remains constant. Thus, measurements of free T3 concentrations correlate more reliably with clinical status than total T3 levels. The increase in total T3 levels associated with pregnancy, oral contraceptives and estrogen therapy result in higher total T3 levels while the free T3 concentration remains basically unchanged.
Gene Symbol fT3
Standard Curve
Representative standard curve for Triiodothyronine, Free (FT3) ELISA
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.