Human Thryoxine, Free (fT4) ELISA kit

CAT#: EA100836

Human Thryoxine, Free (fT4) ELISA kit

Product Images

Representative standard curve for Thryoxine, Free (FT4) ELISA

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 0.4-7.4 ng/dl
• Sample Type: Serum
• Sample Volume: 50 µl/well
• Specificity: This kit is used for quantitative detection human Thryoxine, Free (FT4)
• Sensitivity: 0.4 ng/dl
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwells coated with Streptavidin: 12x8x1
  • 2. fT4 Standard: 6 vials ( ready to use): 0.5 ml
  • 3. Anti-T4 Biotin Solution: 7 ml
  • 4. fT4 Enzyme conjugate: 1 Bottle (ready to use): 7 ml
  • 5. TMB Substrate: 1 bottle (ready to use): 12 ml
  • 6. Stop Solution: 1 bottle (ready to use): 12 ml
  • 7. 20X Wash concentrate: 1 bottle: 25 ml
• Background: Over 99% of thyroxine (T4) circulates in blood is bound to carrier proteins; thyroxine-binding globulin (TBG). However, only the free (unbound) portion of Thyroxine is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alters, the total T4 level changes so that the free T4 concentration remains constant. Thus, measurements of free T4 concentrations correlate more reliably with clinical status than total T4 levels. The increase in total T4 levels associated with pregnancy, oral contraceptives and estrogen therapy result in higher total T4 levels while the free T4 concentration remains basically unchanged.
• Standard Curve:
  
  Representative standard curve for Thryoxine, Free (FT4) ELISA