Human Angiostatin K1-3 ELISA Kit

CAT#: EA100568

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Human Angiostatin K1-3 ELISA Kit



USD 623.00


Availability*
3 Weeks

Size
    • 1 x 96 wells

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Specifications

Product Data
Assay Type Sandwich ELISA kit of Quantitative Detection for Human Angiostatin K1-3
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10,000pg/ml
Sample Type serum, plasma and Cell culture supernates.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human Angiostatin K1-3
Sensitivity <10pg/ml
Reactivities Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- human Angiostatin K1-3 antibody 1
  • Lyophilized recombinant human Angiostatin K1-3 standard 10ng/tubex2
  • Biotinylated anti- human Angiostatin K1-3 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity(ED50 =70 nM) relative to kringles 1-4(ED50 = 135 nM).
Gene Symbol PLG
Gene ID 5340
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.