Human HSP27 ELISA Kit

CAT#: EA100556

Human HSP27 ELISA Kit

 Product Datasheet for 'EA100556'

USD 484.00

3 Weeks

    • 96-Well

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Product Data
Description Human HSP27 ELISA Kit
Size 96-well
Format 8×12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Human HSP27
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 62.5pg/ml-4000pg/ml
Sample Type Cell culture supernates, Cell lysates, serum, plasma(heparin, EDTA) and tissue homogenates.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human HSP27
Sensitivity <5pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
  • 96-well plate precoated with anti- human HSP27 antibody 1
  • Lyophilized recombinant human HSP27 standard 10ng/tubex2
  • Biotinylated anti- human HSP27 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Hsp27 is a chaperone of the sHsp(small heat shock protein) group among ubiquitin, alpha-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7kDa.
Gene Symbol HSPB1
Gene ID 3315
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.