Human ESM1/Endocan ELISA Kit

CAT#: EA100477

Human ESM1/Endocan ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human ESM1/Endocan
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 31.2pg/ml-2000pg/ml
• Sample Type: Cell culture supernates, serum and plasma(heparin, EDTA).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human ESM1
• Sensitivity: <10pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human ESM1 antibody 1
  • Lyophilized recombinant human ESM1 standard 10ng/tubex2
  • Biotinylated anti- human ESM1 antibody 100ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 100ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
  • Plate Sealers 4 x
  • Wash Buffer (25x) 20 ml
• Background: Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF(vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).
• Gene Symbol: ESM1
• Gene ID: 11082