Human Angiopoietin-2 ELISA Kit

CAT#: EA100417

Human Angiopoietin-2 ELISA Kit


 Product Datasheet for 'EA100417'

USD 500.00


Availability*
3 Weeks

Size
    • 96-Well

Product images

Specifications

Product Data
Description Human Angiopoietin-2 ELISA Kit
Size 96-well
Format 8×12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Human Angiopoietin-2
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156pg/ml-10,000pg/ml
Sample Type Cell culture supernates, serum, plasma(heparin, EDTA), saliva and human milk.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human Angiopoietin-2
Sensitivity <10pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- human Angiopoietin-2 antibody 1
  • Lyophilized recombinant human Angiopoietin-2 standard 10ng/tubex2
  • Biotinylated anti- human Angiopoietin-2 antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
  • Required But Not Provided
Background Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. Angiopoietin 1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
Gene Symbol ANGPT2
Gene ID 285

Documents

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