Human CCL21/6Ckine ELISA Kit

CAT#: EA100382

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Human CCL21/6Ckine ELISA Kit



USD 534.00


Availability*
3 Weeks

Size
    • 1 x 96 wells

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Specifications

Product Data
Description Human CCL21/6Ckine ELISA Kit
Size 1 x 96 wells
Assay Type Sandwich ELISA kit of Quantitative Detection for Human CCL21/6Ckine
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 62.5pg/ml-4000pg/ml
Sample Type Cell culture supernates. serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human CCL21
Sensitivity <12pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti-human CCL21 antibody | 1
  • Lyophilized recombinant human CCL21 standard | 10ng/tube x 2
  • Biotinylated anti-human CCL21 antibody | 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100)
  • Sample diluent buffer | 30ml
  • Antibody diluent buffer | 12ml
  • ABC diluent buffer | 12ml
  • TMB color developing agent | 10ml
  • TMB stop solution | 10ml
  • Plate Sealers | 4
  • Wash Buffer (25x) | 20 ml
Background Chemokine(C-C motif) ligand 21(CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine(because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine(SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13(CXCL13), CC chemokine ligand 21(CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.
Gene Symbol CCL21
Gene ID 6366
Other products for "CCL21"
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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