Human CCL4/MIP-1 beta ELISA Kit

CAT#: EA100311

Human CCL4/MIP-1 beta ELISA Kit

Product Images

Representative standard curve for Human CCL4/MIP-1 beta PicoKine ELISA Kit

Specifications

Product Data

• Assay Type: Sandwich ELISA kit of Quantitative Detection for Human CCL4/MIP-1 beta
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 7.8pg/ml-500pg/ml
• Sample Type: Cell culture supernates, serum and plasma(heparin, EDTA, citrate).
• Sample Volume: 100 ul
• Specificity: This kit is used for quantitative detection of human CCL4
• Sensitivity: <1pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 96-well plate precoated with anti- human CCL4/MIP-1 beta antibody 1
  • Lyophilized recombinant human CCL4/MIP-1 beta standard 10ng/tubex2
  • Biotinylated anti- human CCL4/MIP-1 beta antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
• Background: Chemokine(C-C motif) ligand 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. the CCL4 gene was assigned to a slightly more distal location: 17q21-q23 rather than 17q11-q21.
• Gene Symbol: CCL4
• Gene ID: 6351
• Standard Curve:
  
  Representative standard curve for Human CCL4/MIP-1 beta PicoKine ELISA Kit