Human Amphiregulin(AR) ELISA Kit

CAT#: EA100203

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Human Amphiregulin(AR) ELISA Kit



USD 485.00


Availability*
3 Weeks

Size
    • 1 x 96 wells

Product images

Specifications

Product Data
Description Human Amphiregulin(AR) ELISA Kit
Size 1 x 96 wells
Assay Type Sandwich ELISA kit of Quantitative Detection for Human Amphiregulin(AR)
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 15.6pg/ml-1000pg/ml
Sample Type Cell culture supernates, serum, plasma(heparin, EDTA), saliva and human milk.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human Amphiregulin
Sensitivity <10pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- human Amphiregulin antibody 1
  • Lyophilized recombinant human Amphiregulin standard 10ng/tubex2
  • Biotinylated anti- human Amphiregulin antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to 4q13.1. It is an autocrine growth factor as well as a mitogen forastrocytes, Schwann cells, fibroblasts. Amphiregulin is related to toepidermal growth factor(EGF) and transforming growth factor alpha(TGF-alpha). This protein interacts with the Epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.
Gene Symbol AREG
Gene ID 374
Standard Curve
Representative standard curve for Human Amphiregulin(AR) PicoKine ELISA Kit
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