Human sE-selectin ELISA Kit (48-well)

CAT#: EA100111

Human sE-selectin ELISA Kit (48-well)


 Product Datasheet for 'EA100111'

USD 280.00


Availability*
3 Weeks

Size
    • 48-Well

Product images

Specifications

Product Data
Description Human sE-selectin ELISA Kit (48-well)
Size 48-well
Format 48-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Curve Range 780-50000pg/ml
Sample Type Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume 20 uL
Specificity Natural and recombinant Human sE-selectin Ligand
Sensitivity 200pg/mL
Reactivity Human
Interference No significant interference observed with available related molecules.
Background

E-Selectin (also known as Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, or CD62E) is a 115 kDa, type I transmembrane glycoprotein expressed only on endothelial cells and only after activation by inflammatory cytokines (IL-1. or TNF-.) or endotoxins . Expression is transitory, reaching a maximum within about 6 hours of stimulation and then declining with shedding of soluble E-Selectin . Cell-surface E-Selectin is a mediator of the rolling attachment of leukocytes to the endothelium, an essential step in extravasation of leukocytes at the site of inflammation thereby playing a key role in localized inflammatory response. E-Selectin is believed to be particularly important in inflammation involving the skin .

The extracellular part of E-Selectin includes a calcium-dependent C2-type lectin domain, an epidermal growth factor (EGF) domain, and six repeats of a complement-regulatory-protein-like sequence . E-Selectin binds sialyl Lewis X (sLex), a sialic acid-galactose-N-acetylglucosamine-fructose tetrasaccharide, but the actual recognition is thought to be for a specific presentation of those glycosyl units in a precise three-dimensional configuration on a specific glycoprotein rather than for that particular carbohydrate .

Soluble E-Selectin (sE-Selectin) is found in the blood of healthy individuals , probably arising from proteolytic cleavage of the surface-expressed molecule . Elevated levels of sE-Selectin in serum have been reported in a variety of pathological conditions . Although it might be anticipated that sE-Selectin would suppress leukocyte migration by competing with surface-associated E-Selectin, it may actually activate neutrophils and act as a pro-inflammatory agent .

Gene Symbol SELE
Gene ID 6401
Standard Curve
Representative standard curve for sE-selectin ELISA. sE-selectin was diluted in serial two-fold steps in Sample Diluent.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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