Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

CAT#: TA50011-1

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

Product Images

Immunoblot analysis of randomly picked 7 different Myc/DDK tagged overexpression lysates and Myc/DDK tagged Western molecular weight marker (MWM1001) with TA50011 at 1:2,000.

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  Hela cells transiently transfected with Myc/DDK tagged GFAP ORF cDNA clone were immunostained with 4C5 anti-DDK antibody (TA50011, 1:1000), and then colored in green with Alexa 488 conjugated secondary antibody (1:2000). The nuclei (blue) were counterstained with DAPI.

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  Protein purification of a human protein from over-expression lysate with anti-DDK antibody affinity column (TA50011, clone 4C5)

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  HEK293T cells transfected with either Myc/DDK tagged PROM2 ORF overexpression plasmid (red) or empty vector control plasmid (blue) were immunostained with OriGene anti-DDK antibody (TA50011, 1:1000) and analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4C5 (formerly 4C5)
• Applications: FC, IF, IP, WB
• Recommended Dilution: WB: 1:2000, IP: 1:200, IF 1:1000, FC: 1:1000
• Target: DDK (FLAG tag): DYKDDDDK
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide coupled to KLH
• Isotype: IgG2a
• Formulation: PBS (pH7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Storage: Store at -20°C.
• Stability: Stable for 1 year at -20°C from date of shipment.
• Synonyms: ANTI DDK, ANTI-DDK, ANTIDDK, FLAG TAG, ANTI FLAG, ANTI-FLAG, ANTI FLAG ANTIBODY, FLAG ANTIBODY, M2 ANTIBODY, FLAG M2, ANTIBODY FOR FLAG, 4C5, ANTI DDK 4C5, DDK 4C5, ANTI-DDK 4C5, DYKDDDDK TAG, D-TAG ANTIBODY, ANTI D-TAG, ANTI D TAG, ANTI-D TAG, ANTI-MDDK, ANTI-MYC DDK, ANTI-MYC/DDK, MYC/DDK ANTIBODY, MYC DDK ANTIBODY, ANTI MYC DDK, ANTI DDK MYC, ANTI-DDK/MYC, ANTI-DDK MYC, FLAG AB, FLAG MAB, DDK ANTIBODY
• Note: This antibody doesn't recognize N- terminus DDK tag. Please use TA180144 for the detection of DDK tag at any location in Western blotting.