Rat IgG2a (gamma 2a chain specific) Mouse Monoclonal Antibody [Clone ID: 2A 8F4]

CAT#: AM08092RP-N

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, PE

Product Images

Immunofluorescent Staining: BALB/c splenocytes were incubated with Rat IgG1 Isotype Control, Rat anti-Mouse CD22 (2D6, Rat IgG1), Rat anti-Mouse CD8 (53-6.7, Rat IgG2a), Rat anti-Mouse CD45 (LCA) (I3/2.3, Rat IgG2b), Rat anti-Mouse CD24 (30-F1, Rat IgG2c), and Rat anti-Mouse CD45RC (GL24, Rat IgM). After washing, the cells were stained with PE-labeled Mouse Anti-Rat IgG2a. Small Lymphocytes were gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 0.02 µg/10e6 cells.

Specifications

Product Data

• Clone Name: 2A 8F4
• Applications: FC, IF
• Recommended Dilution: Flow Cytometry: < / = 0.1 µg/10e6 cells.
  Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody reacts with the gamma-2a heavy chain (Fc) of Rat IgG2a.
• Formulation: PBS containing 0.09% Sodium Azide as preservative and a stabilizing agent.
  Label: PE
  State: Liquid purified Ig fraction.
  Label: R-Phycoerythrin
• Concentration: lot specific
• Conjugation: PE
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.