AMACR Mouse Monoclonal Antibody [Clone ID: UMAB68]

CAT#: UM870012

AMACR mouse monoclonal antibody,clone UMAB68

Product Images

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AMACR mouse monoclonal antibody. (UM800012; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded of human kidney using anti-AMACR clone UMAB68 mouse monoclonal antibody at 1:200 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM800012 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The image shows strong cytoplsamic staining in the kidney tubual cells.

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  Immunofluorescent staining of HeLa cells using anti-AMACR mouse monoclonal antibody (UM800012, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Specifications

Product Data

• Clone Name: UMAB68
• Applications: IF, IHC
• Recommended Dilution: IHC 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human AMACR (NP_055139) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.2 kDa
• Gene Name: Homo sapiens alpha-methylacyl-CoA racemase (AMACR), transcript variant 1, mRNA.
• Database Link:
  NP_055139
  Entrez Gene 23600 Human
  UniProt ID Q9UHK6
• Background: This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]
• Synonyms: AMACRD; CBAS4; RACE; RM

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Primary bile acid biosynthesis