NSE (ENO2) Mouse Monoclonal Antibody [Clone ID: UMAB287]

CAT#: UM800177CF

Carrier-free (BSA/glycerol-free) NSE(ENO2) mouse monoclonal antibody, clone UMAB287

Product Images

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NSE (ENO2) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800177) (1:15000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NSE (ENO2) mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800177) (1:15000)

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-NSE (ENO2) mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120 oC for 3 min, UM800177) (1:15000)

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NSE (ENO2) (Cat# RC201085, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NSE(ENO2) antibody (Cat# UM800177)(1:1000).

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  OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-NSE (ENO2) mouse monoclonal antibody (UM800177). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. (1:100)

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Specifications

Product Data

• Clone Name: UMAB287
• Applications: IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:15000
• Reactivities: Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 188-293 of human NSE (NP_001966) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Predicted Protein Size: 47.3 kDa
• Gene Name: Homo sapiens enolase 2 (ENO2), mRNA.
• Database Link:
  NP_001966
  Entrez Gene 13807 MouseEntrez Gene 24334 Rat
  UniProt ID P09104
• Background: This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
• Synonyms: HEL-S-279; NSE

Reference Data

• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation