Von Willebrand Factor (VWF) Mouse Monoclonal Antibody [Clone ID: UMAB279]

CAT#: UM800169CF

Carrier-free (BSA/glycerol-free) VWF mouse monoclonal antibody, clone UMAB279

Product images

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-VWF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800169) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-VWF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800169) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-VWF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800169) (1:500)

Specifications

Product Data

• Clone Name: UMAB279
• Applications: IHC
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 764-1263 of human VWF (NP_000543) produced in SF9 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Predicted Protein Size: 309.3 kDa
• Gene Name: Homo sapiens von Willebrand factor (VWF), mRNA.
• Database Link:
  NP_000543
  Entrez Gene 7450 Human
  UniProt ID P04275
• Background: The glycoprotein encoded by this gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008].
• Synonyms: F8VWF; VWD

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, ECM-receptor interaction, Focal adhesion