CD8A Mouse Monoclonal Antibody [Clone ID: UMAB241]

CAT#: UM800133CF

Carrier-free (BSA/glycerol-free) CD8A mouse monoclonal antibody,clone UMAB241

Product Images

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CD8A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800133) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD8A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800133) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-CD8A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800133) (1:200).

Specifications

Product Data

• Clone Name: UMAB241
• Applications: IHC
• Recommended Dilution: IHC 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 22-182 of human CD8A (NP_001759) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.67 kDa
• Gene Name: Homo sapiens CD8a molecule (CD8A), transcript variant 1, mRNA.
• Database Link:
  NP_001759
  Entrez Gene 925 Human
  UniProt ID P01732
• Synonyms: CD8; Leu2; MAL; p32

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway