AMACR Mouse Monoclonal Antibody [Clone ID: UMAB215]

CAT#: UM800107CF

Carrier-free (BSA/glycerol-free) AMACR mouse monoclonal antibody,clone UMAB215

Product Images

Immunohistochemical staining of paraffin-embedded human kidney tissue within the normal limits using anti-AMACR mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800107 was diluted 1:800 and detection was done with HRP secondary and DAB chromogen. Weak cytoplasmic and membraneous stain was seen in the tubule epithelial cells.

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  Immunohistochemical staining of paraffin-embedded carcinoma of human liver tissue using anti-AMACR mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800107 was diluted 1:800 and detection was done with HRP secondary and DAB chromogen. Strong cytoplasmic and membraneous stain was seen in tumor cells.

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  Immunohistochemical staining of paraffin-embedded carcinoma of human prostate tissue using anti-AMACR mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800107 was diluted 1:800 and detection was done with HRP secondary and DAB chromogen. Strong cytoplasmic and membraneous stain was seen in tumor cells.

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  OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-AMACR mouse monoclonal antibody (UM800107). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Specifications

Product Data

• Clone Name: UMAB215
• Applications: 10k-ChIP, IHC
• Recommended Dilution: IHC 1:100~800
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human AMACR (NP_055139) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.2 kDa
• Gene Name: Homo sapiens alpha-methylacyl-CoA racemase (AMACR), transcript variant 1, mRNA.
• Database Link:
  NP_055139
  Entrez Gene 23600 Human
  UniProt ID Q9UHK6
• Background: This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]
• Synonyms: AMACRD; CBAS4; RACE; RM

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Primary bile acid biosynthesis