Nkx3.1 (NKX3-1) Mouse Monoclonal Antibody [Clone ID: UMAB195]

CAT#: UM800087

NKX3-1 mouse monoclonal antibody,clone UMAB195

Product Images

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-NKX3-1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, UM800087) (1:200)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NKX3-1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, UM800087) (1:200)

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  Immunohistochemical staining of 3 cases of paraffin-embedded human endometrial carcinoma using anti-NKX3.1 clone UMAB195 mouse monoclonal antibody (UM800087) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI ACCEL pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. No staining seen in the tumor cells but some cytoplasmic staining was visualized in the third case along with weak nuclear staining.

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  OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-NKX3-1 mouse monoclonal antibody (UM800087). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Specifications

Product Data

• Clone Name: UMAB195
• Applications: 10k-ChIP, IHC
• Recommended Dilution: IHC 1:100~200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NKX3-1 (NP_006158) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.2 kDa
• Gene Name: NK3 homeobox 1
• Database Link:
  NP_006158
  Entrez Gene 4824 Human
  UniProt ID Q99801
• Background: This gene encodes a homeobox-containing transcription factor. This transcription factor functions as a negative regulator of epithelial cell growth in prostate tissue. Aberrant expression of this gene is associated with prostate tumor progression. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jan 2012]
• Synonyms: BAPX2; NKX3; NKX3.1; NKX3A

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Prostate cancer