Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody [Clone ID: UMAB172]

CAT#: UM800065CF

Carrier-free (BSA/glycerol-free) KRT20/CK20 mouse monoclonal antibody,clone UMAB172

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KRT20 mouse monoclonal antibody. (UM800065; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• 

  Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

• 

  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.

• 

  OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-KRT20 mouse monoclonal antibody (UM800065). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Specifications

Product Data

• Clone Name: UMAB172
• Applications: 10k-ChIP, IHC, WB
• Recommended Dilution: IHC 1:100~200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human KRT20 (NP_061883) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48.3 kDa
• Gene Name: Homo sapiens keratin 20 (KRT20), mRNA.
• Database Link:
  NP_061883
  Entrez Gene 54474 Human
  UniProt ID P35900
• Background: The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
• Synonyms: CD20; CK-20; CK20; K20; KRT21