Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: UMAB160]

CAT#: UM800055CF

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Carrier-free (BSA/glycerol-free) Vimentin (VIM) mouse monoclonal antibody,clone UMAB160



  View other "UMAB160" antibodies (2)

USD 614.00


Availability*
4 Weeks

Size
    • 100 ug


Specifications

Product Data
Clone Name UMAB160
Applications IF, IHC, WB
Recommended Dilution IHC 1:500
Reactivity Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human VIM (NP_003371) produced in E.coli.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 53.5 kDa
Gene Name Homo sapiens vimentin (VIM), mRNA.
Background This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract. [provided by RefSeq, Jun 2009]
Synonyms CTRCT30; HEL113
Reference Data
Protein Families ES Cell Differentiation/IPS

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.