NY-ESO-1 (CTAG1B) Mouse Monoclonal Antibody [Clone ID: UMAB72]

CAT#: UM500060CF

Carrier-free (BSA/glycerol-free) CTAG1B (NY-ESO-1) mouse monoclonal antibody, clone UMAB72

Product Images

Immunohistochemical staining of paraffin-embedded Human testicular cancer tissue using anti-CTAG1B mouse monoclonal antibody. (UM500060; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTAG1B (RC213318, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTAG1B (1:500).

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  Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CTAG1B monoclonal antibody (1:500).

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

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  Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY CTAG1B (RC213318) using anti-CTAG1B antibody (UM500060/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).

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  Flow cytometric analysis of living 293T cells transfected with CTAG1B overexpression plasmid (RC213318), Red)/empty vector (PS100001, Blue) using anti-CTAG1B antibody (UM500060). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

• Flow cytometric analysis of living NCI-H1299 cells, using anti-CTAG1B antibody (UM500060, Red), compared to an isotype control (green) (1:100).

• Flow cytometric analysis of living A375 cells, using anti-CTAG1B antibody (UM500060, Red), compared to an isotype control (green) (1:100).

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CTAG1B mouse monoclonal antibody (UM500060). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB72
• Applications: 10k-ChIP, FC, IF, IHC, WB
• Recommended Dilution: IHC 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CTAG1B (NP_001318) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17.8 kDa
• Gene Name: cancer/testis antigen 1B
• Database Link:
  NP_001318
  Entrez Gene 1485 Human
  UniProt ID P78358
• Background: The protein encoded by this gene is an antigen that is overexpressed in many cancers but that is also expressed in normal testis. This gene is found in a duplicated region of the X-chromosome and therefore has a neighboring gene of identical sequence. [provided by RefSeq, Jan 2012]
• Synonyms: CT6.1; CTAG; CTAG1; ESO1; LAGE-2; LAGE2B; NY-ESO-1

Reference Data

• Protein Families: Druggable Genome