RACGAP1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3D8]

CAT#: TA811651AM

RACGAP1 mouse monoclonal antibody,clone OTI3D8, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RACGAP1 (RC226045, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RACGAP1 (1:2000). Positive lysates LY426645 (100ug) and LC426645 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-RACGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811651) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human gastric stromal tumor tissue using anti-RACGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811651) (1:500)

Specifications

Product Data

• Clone Name: OTI3D8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 123-238 of human RACGAP1 (NP_001119575) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Rac GTPase activating protein 1
• Database Link:
  NP_001119575
  Entrez Gene 26934 MouseEntrez Gene 315298 RatEntrez Gene 29127 Human
  UniProt ID Q9H0H5
• Background: This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016]
• Synonyms: CYK4; HsCYK-4; ID-GAP; MgcRacGAP

Reference Data

• Protein Families: Druggable Genome