AIF (AIFM1) Mouse Monoclonal Antibody [Clone ID: OTI4E6]

CAT#: TA811161

AIFM1 mouse monoclonal antibody,clone OTI4E6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY AIFM1 (Cat# RC225349, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AIFM1 (Cat# TA811161)(1:2000).

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  Western blot analysis of extracts (35ug) from 6 different cell lines and mouse brain tissue lysate by using anti-AIFM1 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and AIFM1-Knockout 293T cells (KO, Cat# LC811853) were separated by SDS-PAGE and immunoblotted with anti-AIFM1 monoclonal antibody TA811161, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-AIFM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811161) (1:500)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AIFM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811161) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AIFM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811161) (1:500)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AIFM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811161) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AIFM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811161) (1:500)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-AIFM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811161) (1:500)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AIFM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811161) (1:500)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-AIFM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811161) (1:500)

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Specifications

Product Data

• Clone Name: OTI4E6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 134-483 of human AIFM1 (NP_001124318) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: apoptosis inducing factor, mitochondria associated 1
• Database Link:
  NP_001124318
  Entrez Gene 26926 MouseEntrez Gene 83533 RatEntrez Gene 9131 Human
  UniProt ID O95831
• Synonyms: AIF; CMT2D; CMTX4; COWCK; COXPD6; NADMR; NAMSD; PDCD8

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Apoptosis