ESE1 (ELF3) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI6G6]

CAT#: TA809989AM

ELF3 mouse monoclonal antibody,clone OTI6G6, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ELF3 (Cat# RC225564, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELF3 (Cat# TA809989)(1:2000). Positive lysates LY426477 (100ug) and LC426477 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ELF3 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ELF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809989) (1:250)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ELF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809989) (1:250)

Specifications

Product Data

• Clone Name: OTI6G6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:250
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 175-286 of human ELF3(NP_001107781) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.3 kDa
• Gene Name: E74 like ETS transcription factor 3
• Database Link:
  NP_001107781
  Entrez Gene 13710 MouseEntrez Gene 304815 RatEntrez Gene 1999 Human
  UniProt ID P78545
• Synonyms: EPR-1; ERT; ESE-1; ESX

Reference Data

• Protein Families: Transcription Factors