SMC1 (SMC1A) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2D12]

CAT#: TA809828BM

SMC1A mouse monoclonal antibody,clone OTI2D12, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMC1A (RC215888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMC1A (1:500). Positive lysates LY401901 (100ug) and LC401901 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SMC1A monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SMC1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809828) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SMC1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809828) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SMC1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809828) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-SMC1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809828) (1:500)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SMC1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809828) (1:500)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SMC1A mouse monoclonal antibody. This figure shows negative staining. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809828) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SMC1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809828) (1:500)

• Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-SMC1A mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA (1:500)

Show all 10 images

Specifications

Product Data

• Clone Name: OTI2D12
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 889-1016 of human SMC1A (NP_006297) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 143.1 kDa
• Gene Name: structural maintenance of chromosomes 1A
• Database Link:
  NP_006297
  Entrez Gene 24061 MouseEntrez Gene 63996 RatEntrez Gene 8243 Human
  UniProt ID Q14683
• Synonyms: CDLS2; DXS423E; SB1.8; SMC1; SMC1alpha; SMC1L1; SMCB

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis