alpha 1 Fetoprotein (AFP) Mouse Monoclonal Antibody [Clone ID: OTI4D8]

CAT#: TA809083

AFP mouse monoclonal antibody, clone OTI4D8 (formerly 4D8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (RC206622, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP (1:2000). Positive lysates LY400455 (100ug) and LC400455 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AFP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809083) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AFP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809083) (1:150)

• 

  Immunofluorescent staining of HT29 cells using anti-AFP mouse monoclonal antibody (TA809083) (1:100).

Specifications

Product Data

• Clone Name: OTI4D8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-397 of human AFP (NP_001125) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 66.3 kDa
• Gene Name: alpha fetoprotein
• Database Link:
  NP_001125
  Entrez Gene 174 Human
  UniProt ID P02771
• Background: This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]
• Synonyms: AFPD; FETA; HPAFP

Reference Data

• Protein Families: Druggable Genome, Secreted Protein