EPLIN (LIMA1) Mouse Monoclonal Antibody [Clone ID: OTI1A3]

CAT#: TA808537S

LIMA1 mouse monoclonal antibody, clone OTI1A3 (formerly 1A3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LIMA1 (Cat# RC211066, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIMA1 (Cat# TA808537)(1:2000). Positive lysates LY402544 (100ug) and LC402544 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LIMA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808537) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LIMA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808537) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-LIMA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808537) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-LIMA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808537) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LIMA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808537) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LIMA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808537) (1:150)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-LIMA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808537) (1:150)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-LIMA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808537) (1:150)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-LIMA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808537) (1:150)

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Specifications

Product Data

• Clone Name: OTI1A3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-275 of human LIMA1(NP_057441) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85 kDa
• Gene Name: LIM domain and actin binding 1
• Database Link:
  NP_057441
  Entrez Gene 51474 Human
  UniProt ID Q9UHB6
• Background: This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011]
• Synonyms: EPLIN; SREBP3