Lymphocyte Activation Gene 3 (LAG3) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI5C3]

CAT#: TA807073BM

LAG3 mouse monoclonal antibody, clone OTI5C3 (formerly 5C3), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAG3 (RC220269, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAG3. Positive lysates LY400828 (100ug) and LC400828 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 human tissue by using anti-LAG3 monoclonal antibody at 1:200 dilution.

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  Western blot analysis of extracts (35ug) from 2 cell lines (Human PBMC treated with 10ug/ml PHA for 72h (Right)/untreated (Left)) lysates by using anti-LAG3 monoclonal antibody (1:150).

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  Flow cytometric analysis of living 293T cells transfected with LAG3 overexpression plasmid (RC220269), Red)/empty vector (PS100001, Blue) using anti-LAG3 antibody (TA807073). Cells incubated with a non-specific antibody (Green) were used as isotype control. (1;100)

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  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-LAG3 antibody (TA807073) (1:100).

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  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right) using anti-LAG3 antibody (TA807073). Cells incubated with a non-specific antibody (Left) were used as isotype control (1:100).

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Specifications

Product Data

• Clone Name: OTI5C3
• Applications: FC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 66-332 of human LAG3(NP_002277) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.8 kDa
• Gene Name: lymphocyte activating 3
• Database Link:
  NP_002277
  Entrez Gene 3902 Human
  UniProt ID P18627
• Background: Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]
• Synonyms: CD223

Reference Data

• Protein Families: Transmembrane