RET Mouse Monoclonal Antibody [Clone ID: OTI1G1]

CAT#: TA805761S

RET mouse monoclonal antibody, clone OTI1G1 (formerly 1G1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RET (Cat# RC214268, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET (Cat# TA805761)(1:500).

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-RET monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805761) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of RET positive Human lung tissue using anti-RET mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805761) (1:150)

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  Immunofluorescent staining of TT cells using anti-RET antibody (TA805761/green, upper left; DAPI/blue, lower left; MERGED, upper right) or Isotype control (MERGED, lower right) (1:100).

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Specifications

Product Data

• Clone Name: OTI1G1
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 713-1017 of human RET(NP_066124) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 124.1 kDa
• Gene Name: ret proto-oncogene
• Database Link:
  NP_066124
  Entrez Gene 24716 RatEntrez Gene 5979 Human
  UniProt ID P07949
• Background: This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq, Jul 2008]
• Synonyms: CDHF12; CDHR16; HSCR1; MEN2A; MEN2B; MTC1; PTC; RET-ELE1; RET51

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Endocytosis, Pathways in cancer, Thyroid cancer