Natriuretic Peptide Receptor A (NPR1) Mouse Monoclonal Antibody [Clone ID: OTI7H7]

CAT#: TA805600

NPR1 mouse monoclonal antibody, clone OTI7H7 (formerly 7H7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NPR1 (Cat# RC209267, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPR1(Cat# TA805600). Positive lysates LY424461 (100ug) and LC424461 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI7H7
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 746-1006 of human NPR1 (NP_000897) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 118.7 kDa
• Gene Name: natriuretic peptide receptor 1
• Database Link:
  NP_000897
  Entrez Gene 18160 MouseEntrez Gene 24603 RatEntrez Gene 4881 Human
  UniProt ID P16066
• Background: Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 [PubMed 7982997]). The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961). Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively). [supplied by OMIM, May 2009]
• Synonyms: ANPa; ANPRA; GUC2A; GUCY2A; NPRA

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Purine metabolism, Vascular smooth muscle contraction