BRCA1 Mouse Monoclonal Antibody [Clone ID: OTI5D10]

CAT#: TA802618

BRCA1 mouse monoclonal antibody, clone OTI5D10 (formerly 5D10)

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA802618) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA802618) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA802618) (1:200)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (TA802618) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA802618) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA802618) (1:200)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA802618) (1:200)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA802618) (1:200)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA802618) (1:200)

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Specifications

Product Data

• Clone Name: OTI5D10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1151-1473 of human BRCA1 (NP_009225) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BRCA1, DNA repair associated
• Database Link:
  NP_009225
  Entrez Gene 672 Human
  UniProt ID P38398
• Background: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]
• Synonyms: BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Ubiquitin mediated proteolysis