Kallikrein 2 (KLK2) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI5D6]

CAT#: TA802077AM

KLK2 mouse monoclonal antibody,clone 5D6, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY KLK2 (Cat# RC202667, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLK2(Cat# TA802077). Positive lysates LY417229 (100ug) and LC417229 (20ug) can be purchased separately from OriGene.

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  Western blot analyses for the cross-reactivity between KLK2 and KLK3 overexpression lysate. The results indicate that KLK3 overexpressed lysate is not getting detected by the anti-KLK2 antibody (TA802077)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KLK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802077)

Specifications

Product Data

• Clone Name: OTI5D6
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 25-261 of human KLK2 (NP_005542) produced in SF9 cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.5 kDa
• Gene Name: kallikrein related peptidase 2
• Database Link:
  NP_005542
  Entrez Gene 3817 Human
  UniProt ID P20151
• Background: This gene encodes a member of the grandular kallikrein protein family. Kallikreins are a subgroup of serine proteases that are clustered on chromosome 19. Members of this family are involved in a diverse array of biological functions. The protein encoded by this gene is a highly active trypsin-like serine protease that selectively cleaves at arginine residues. This protein is primarily expressed in prostatic tissue and is responsible for cleaving pro-prostate-specific antigen into its enzymatically active form. This gene is highly expressed in prostate tumor cells and may be a prognostic maker for prostate cancer risk. Alternate splicing results in both coding and non-coding transcript variants. [provided by RefSeq, Jan 2012]
• Synonyms: hGK-1; hK2; KLK2A2

Reference Data

• Protein Families: Druggable Genome, Protease