p95 NBS1 (NBN) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3A4]

CAT#: TA801422BM

NBN mouse monoclonal antibody,clone 3A4, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NBN (RC214682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NBN. Positive lysates LY419300 (100ug) and LC419300 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801422)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NBN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801422)

Specifications

Product Data

• Clone Name: OTI3A4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 183-460 of human NBN (NP_002476) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84.8 kDa
• Gene Name: nibrin
• Database Link:
  NP_002476
  Entrez Gene 4683 Human
  UniProt ID O60934
• Background: Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]
• Synonyms: AT-V1; AT-V2; ATV; NBS; NBS1; P95

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination