Cullin 4B (CUL4B) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI7F8]

CAT#: TA800411AM

CUL4B mouse monoclonal antibody,clone 7F8, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CUL4B (RC206935, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CUL4B. Positive lysates LY418558 (100ug) and LC418558 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CUL4B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Specifications

Product Data

• Clone Name: OTI7F8
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-300 of human CUL4B (NP_003579) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 103.8 kDa
• Gene Name: cullin 4B
• Database Link:
  NP_003579
  Entrez Gene 72584 MouseEntrez Gene 302502 RatEntrez Gene 492102 DogEntrez Gene 696410 MonkeyEntrez Gene 8450 Human
  UniProt ID Q13620
• Background: This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2

Reference Data

• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis