Lipin 2 (LPIN2) Rabbit Polyclonal Antibody

CAT#: TA590249

Rabbit Polyclonal Lipin 2 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LPIN2 (Cat# RC224070, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LPIN2(Cat# TA590249). Positive lysates LY402358 (100ug) and LC402358 (20ug) can be purchased separately from OriGene.

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  Western Blot: Lipin 2 Antibody - Western Blot was performed using affinity purified SEQer LPIN2 antibody, aa (351-450) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer LPIN2 antibody, aa (351-450) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.

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  ELISA: Lipin 2 Antibody

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LPIN2 Rabbit Polyclonal antibody. (TA590249)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-LPIN2 Rabbit Polyclonal antibody. (TA590249)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-LPIN2 Rabbit Polyclonal antibody. (TA590249)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-LPIN2 Rabbit Polyclonal antibody. (TA590249)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-LPIN2 Rabbit Polyclonal antibody. (TA590249)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LPIN2 Rabbit Polyclonal antibody. (TA590249)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-LPIN2 Rabbit Polyclonal antibody. (TA590249)

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Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody is specific for the Middle Region of the target protein.
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 0.98mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: lipin 2
• Database Link:
  NP_055461
  Entrez Gene 9663 Human
  UniProt ID Q92539
• Background: Lipin 2 plays important roles in controlling the metabolism of fatty acids at differents levels. Lipin 2 acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Lipin 2 also acts also as a nuclear transcriptional coactivator for PPARGC1A to modulate lipid metabolism By similarity.Defects in LPIN2 are the cause of Majeed syndrome (MAJEEDS) [MIM:609628]. An autosomal recessive syndrome characterized by chronic recurrent multifocal osteomyelitis that is of early onset with a lifelong course, congenital dyserythropoietic anemia that presents as hypochromic, microcytic anemia during the first year of life and ranges from mild to transfusion-dependent, and transient inflammatory dermatosis, often manifesting as Sweet syndrome (neutrophilic skin infiltration).
• Synonyms: KIAA0249
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT