Apc11 (ANAPC11) Mouse Monoclonal Antibody [Clone ID: OTI3F5]

CAT#: TA506323

ANAPC11 (Apc11) mouse monoclonal antibody, clone OTI3F5 (formerly 3F5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANAPC11 (RC200097, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANAPC11. Positive lysates LY424181 (100ug) and LC424181 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ANAPC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506323)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ANAPC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506323)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ANAPC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506323)

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  Western blot analysis of extracts (10ug) from COS7 cell line by using anti-ANAPC11 monoclonal antibody at 1:200 dilution.

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Specifications

Product Data

• Clone Name: OTI3F5
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ANAPC11(NP_001002244) produced in HEk293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.5 kDa
• Gene Name: anaphase promoting complex subunit 11
• Database Link:
  NP_001002244
  Entrez Gene 66156 MouseEntrez Gene 498030 RatEntrez Gene 51529 Human
  UniProt ID Q9NYG5
• Synonyms: APC11; Apc11p; HSPC214

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis