BBOX1 Mouse Monoclonal Antibody [Clone ID: OTI4F9]

CAT#: TA505753

BBOX1 mouse monoclonal antibody, clone OTI4F9 (formerly 4F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BBOX1 (Cat# RC203156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BBOX1(Cat# TA505753). Positive lysates LY418299 (100ug) and LC418299 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505753)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505753)

• 

  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505753)

• 

  Anti-BBOX1 mouse monoclonal antibody (TA505753) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BBOX1 (RC203156).

• 

  Immunofluorescent staining of HeLa cells using anti-BBOX1 mouse monoclonal antibody (TA505753).

Show all 6 images

Specifications

Product Data

• Clone Name: OTI4F9
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BBOX1(NP_003977) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.5 kDa
• Gene Name: gamma-butyrobetaine hydroxylase 1
• Database Link:
  NP_003977
  Entrez Gene 170442 MouseEntrez Gene 8424 Human
  UniProt ID O75936
• Background: This gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. [provided by RefSeq, Jul 2008]
• Synonyms: BBH; BBOX; G-BBH; gamma-BBH

Reference Data

• Protein Pathways: Lysine degradation