EIF5A2 Mouse Monoclonal Antibody [Clone ID: OTI6G7]

CAT#: TA505100

EIF5A2 mouse monoclonal antibody, clone OTI6G7 (formerly 6G7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY EIF5A2 (Cat# RC206249, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF5A2(Cat# TA505100). Positive lysates LY412495 (100ug) and LC412495 (20ug) can be purchased separately from OriGene.

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  Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human EIF5A2 plasmid (RC206249, hEIF5A2), human EIF5A1 plasmid (RC200770, hEIF5A1) using anti-EIF5A2 antibody TA505100(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-EIF5A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505100)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EIF5A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505100)

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  Anti-EIF5A2 mouse monoclonal antibody (TA505100) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EIF5A2 (RC206249).

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  HEK293T cells transfected with either RC206249 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EIF5A2 antibody (TA505100), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI6G7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EIF5A2(NP_065123) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.6 kDa
• Gene Name: eukaryotic translation initiation factor 5A2
• Database Link:
  NP_065123
  Entrez Gene 208691 MouseEntrez Gene 310261 RatEntrez Gene 56648 Human
  UniProt ID Q9GZV4
• Synonyms: EIF-5A2; eIF5AII