Frataxin (FXN) Mouse Monoclonal Antibody [Clone ID: OTI1C12]

CAT#: TA504254

FXN (Frataxin) mouse monoclonal antibody, clone OTI1C12 (formerly 1C12)

Product images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FXN (Cat# RC204880, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FXN(Cat# TA504254). Positive lysates LY400054 (100ug) and LC400054 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504254).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504254).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-FXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504254).

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504254).

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504254)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504254)

• Anti-FXN mouse monoclonal antibody (TA504254) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FXN (RC204880).

• HEK293T cells transfected with either RC204880 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FXN antibody (TA504254), and then analyzed by flow cytometry.

• HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FXN (RC204880, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FXN. Positive lysates LY400054 (100ug) and LC400054 (20ug) can be purchased separately from OriGene.

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Specifications

Product Data

• Clone Name: OTI1C12
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FXN(NP_000135) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.47 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.8 kDa
• Gene Name: frataxin
• Database Link:
  NP_000135
  Entrez Gene 2395 Human
  UniProt ID Q16595
• Background: This nuclear gene encodes a mitochondrial protein which belongs to FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in Friedreich ataxia. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: CyaY; FA; FARR; frataxin; FRDA; MGC57199; X25

Reference Data

• Protein Families: Druggable Genome